Description
The single molecule setup uses a confocal microscope for optical characterization of solutions and surfaces down to a single molecule resolution. It consists of a microscope (Nikon) coupled to a continuous-wave (CW). Excitation is available at multiple wavelengths in the visible region from a CW argon/krypton laser. These instruments can monitor a number of fluorescent properties on the surface including fluorescent anisotropy and multiple wavelengths. The fluorescence detection range is 450–850 nm. The spatial resolution of this system is less than 500 nm. Fluorescence correlation spectroscopy can be measured using a correlator with 25 ns time resolution.
Techniques
- Fluorescence correlation spectroscopy
- Fluorescence confocal scan
ASU Unit
Knowledge Enterprise
Rates
| Service | ASU rate | Nonprofit/other academic rate | Private/Industry | Notes |
|---|---|---|---|---|
| Equipment use (base time 2 hours) | $19.25/hr | $24.07/hr | $48.13 | Instrument start-up fee $20 |