Capabilities

This unique combination of resources provides researchers all the tools needed to conduct tissue, cell and stem cell culture-based research. The RegenMed Facility at ASU offers the following services and applications.

PCR

• qPCR – real time PCR for a broad range of applications, including gene expression, genetic variation, and gene regulation
• Thermocyclers
• Nanodrop One and Qbit 3.0 Fluorometer for quantification

Gel Doc Scanner

• DNA/RNA analysis
• Chemiluminescence imaging
• Fluorescence imaging
• Colorimetric imaging
• Analysis of nucleic acid and protein gels, blots, and macroarrays
• Illumination control: trans-UV (302nm), trans white, epi-white
• 480BP70 62 mm, for Hoechst/coumarin
• CCD camera with image resolution of 4 mega pixels, 4.65 x 4.65 µm pixel size

ThermoScientific Heraeus MegaFuge 16R and 8R

• 50ml, 15ml, culture plate rotors
• Megafuge 8R can reach maximum speeds of 17,850 RPM and maximum RCF of 30,279 x g (with fixed angle rotor). This refrigerated centrifuge has a temperature range of -10° to +40°C
• The Megafuge 16R can reach maximum speeds of 15,200 RPM and maximum RCF of 25,830 x g

BioTek Synergy H1 plate reader

• Multimode microplate reader with top and bottom fluorescence intensity
• UV-visible absorbance
• Luminescence
• Fluorescence polarization
• TRF and TR-FRET detection
• Temperature control to 45ºC and shaking
• Available filters include BLUE/GREEN, GRN FP VSBL K, and HTRF

Leica DMi8 inverted fluorescent microscope

• Inverted Epi-fluorescent microscope with a 5, 10, 20 and 40x LWD Phase objectives and DAPI, FITC, TxRed and Cy5 filters
• DFC345 FX monochrome camera
• Image tiling

Olympus/Evident VS200 Slide Scanner

• Brightfield color [H&E, DAB, various stains], Polarized light, Darkfield, Fluorescence with five channels: DAPI/FITC/TRITC/Cy5/Cy7
• Objectives: 2x, 4x, 10x, 20x, 40x [Dry] and 60x [Oil]
• Hamamatsu sCMOS Orca Fusion and a CMOS color camera

Cell culture facilities

• 1300 Series A2 Class II, Type A2 Biological Safety Cabinets
• VWR® Air Jacketed CO2 Incubators
• Mycoplasma testing

Histology services

• ICC/IHC processing
• FFPE [formalin fixed paraffin embedding]
• Cryostat or Microtome sectioning
  

Storage availability at -150C and -80C for boxes or racks

This is a sample list of applications available within the core. Please contact [email protected] for any questions regarding additional capabilities.