RMBF - Leica SP8 White Light Laser Confocal with Light Sheet

Location
LSC – Life Sciences Center C Wing, L2-74
Description

The Leica SP8 White Light Laser Confocal/Light sheet inverted microscope system is designed for confocal live and fixed cell imaging. It is equipped with a white light tunable laser as well as an Argon [488nm] and 405nm lasers. The system has a combination of PMT and HyD GaAsP detectors as well as a DIC bright field detector. The system allows for standard confocal imaging with our without additional resonant scanning speed or can be switched into live cell mode by adding the Tokai Hit stage top incubation chamber and/or Light Sheet module and objectives.

Technical specifications 

  • DMi8 CS Bino DLS inverted fluorescent microscope
  • Super Z galvo stage with tiling capability 
  • Argon laser with 458, 476, 488, 496, and 514nm laser excitation lines 
  • 405nm laser for DAPI excitation
  • White light laser tunable between 470-670nm 
  • Transmitted light bright field detector with DIC
  • Three spectral HyD GaAsP detectors
  • Two spectral PMTs
  • Tandem Scanner 8 kHz
  • Leica Digital Light Sheet with a Hamamatsu Orca Flash 4.0 V2 CMOS camera (2048 x 2048 pixels)
  • Tokai Hit Environmental stage incubation chamber 
  • HyVo2 Deconvolution software – SVI Huygens integrated
  • LAS X Electrophysio9logy Trigger system and software

Objectives on the system (Additional objectives are also available)

  • HCX PL APO CS 10x/0.4NA, Air, 2.2mm WD
  • HC PL APO 20x/0.75 IMM CORR CS2, water/gly/oil, 0.67mm WD
  • HC PL APO 40x/1.3 Oil CS2, 0.24mm WE
  • HC PL APO 63x/1.4oil CS2, 0.14mm WD
  • HC PL FLUOTAR 5x/0.15, Air [light sheet], 12 mm WD
  • HC PL FLUOTAR 2.5/0.07, Air [light sheet], 9.2mm WD

Additional light sheet objectives

  • L 1.6x/0.05 IMM DLS
  • HC PL FLUOTAR 5x/0.15 IMM DLS, water/gly, 4.95mm WD
  • HC APO L10x/0.3 W DLS, Water, 3.6mm WD
  • HC FLUOTAR L25x/0.95 W DLS, Water, 2.5mm WD
Techniques
  • light sheet
  • FRET
  • FRAP
  • Live-cell imaging
  • Tiling
  • Lambda scanning
  • image processing and analysis
  • resonant scanner mode capable of 28fps at 512 x 512
  • photon counting/gating
  • SVI Huygens deconvolution and 3D rendering
  • electrophysiology trigger
Documents and manuals
ASU Unit
Knowledge Enterprise
Rates
Service ASU rate Nonprofit/other academic rate Notes
Unassisted use $35/hour $70.70/hour Maximum 4 hour charge per day 
Technical assistance $50/hour $62/hour  
Materials: frosted glass slides     $10/box     $10/box     Double frosted micro slides used in various microscopy applications. A box includes 72 slides measuring 3” x 1”.
Materials: Mat Tek 35mm dishes $15/set $15/set Sterile coverslip bottom petri dishes for live cell imaging. A set includes 10 dishes. 
Photos
Leica SP8 White Light Laser Confocal with Light Sheet
Videos