Sanger Sequencing
Service |
ASU rate |
Nonprofit/other academic rate |
Notes |
Clean and load |
$3/sample |
$3.10/sample |
For samples already cycled with BigDye |
PCR clean with sequencing |
$4.45/sample |
$5/sample |
For samples needing PCR cleanup |
PCR clean with sequencing, batch rate (49+) |
$3.75/sample |
$4.30/sample |
|
Standard Sequencing |
$3.70/sample |
$4/sample |
For clean samples |
Standard Sequencing, batch rate (49+) |
$3/sample |
$3.30/sample |
NGS Sample Prep
Service |
ASU rate |
Nonprofit/other academic rate |
Notes |
Single cell RNA-seq library preparation |
$2,200/sample |
$2,395/sample |
On 10X Genomics platform |
DNA library preparation |
$150/sample $120/sample $95/sample |
$165/sample $135/sample $110/sample |
Synthesis of NGS libraries from DNA, 100-1000bp long |
Microbiome library preparation |
$65/sample $60/sample $50/sample $30/sample |
$71.50/sample $66.50/sample $56.50/sample $36.50/sample |
Library preparation for microbiome sequencing, starting from genomic DNA; can select either 16S (bacterial), 18S (eukaryotic), or ITS (fungal) regions |
ITS2 | $10/sample | $10/sample | additional ITS (fungal) region option |
cDNA Synthesis only |
$160/sample |
$176/sample |
cDNA synthesis from total RNA using SPIA random hexamers |
RNA library preparation |
$120/sample $90/sample $70/sample |
$132/sample $102/sample $82/sample |
NGS library construction from total RNA, rRNA depletion, or mRNA pulldown |
mRNA pulldown |
$40/sample |
$44/sample |
mRNA pull-down only |
Bacterial rRNA depletion |
$50/sample |
$55/sample |
removal of rRNA from bacterial samples |
Human/Mouse/Rate rRNA depletion | $75/sample | $82.50/sample | removal of rRNA from human, mouse, or rat samples |
Specialty rRNA depletion | $120/sample | $132/sample | removal of rRNA from other species |
|
Instrument training and use
Service |
ASU rate |
Nonprofit/other academic rate |
Notes |
Covaris M220 shearing performed by facility technicians |
$15/sample |
$16.50/sample |
|
Researcher use of the Covaris M220 using consumables provided by the core |
$12/sample |
$13.20/sample |
|
Researcher use of the Covaris M220 using researcher-provided consumables |
$4/half hour |
$6/half hour |
|
Covaris M220 training |
$75/training |
$85/training |
Hands on training – required before using Covaris independently. Includes consumables for 2 samples. |
Agilent TapeStation analysis performed by facility technicians |
$11/sample |
$12/sample |
|
Researcher use of the Agilent TapeStation using consumables provided by the core |
$8/sample |
$9/sample |
|
Researcher use of the Agilent TapeStation using researcher-provided consumables |
$4/half hour |
$6/half hour |
|
Agilent TapeStation training |
$75/training |
$85/training |
Hands on training – required before using Agilent TapeStation independently. Includes consumables for two samples. |
qPCR targeting Illumina adapter regions, in triplicate at three dilutions, with analysis run by facility staff |
$20/sample |
$22/sample |
Run on the QuantStudio5. |
Any researcher qPCR run with researcher-provided consumables |
$15/plate |
$16.50/plate |
Run on the QuantStudio5. |
QuantStudio5 training |
$75/training |
$85/training |
Hands on training – required before using QuantStudio5 independently. Includes consumables for two samples. |
Researcher use of the Qubit using facility-provided consumables for DNA analysis |
$1.50/sample |
$1.75/sample |
Run on the ThermoFisher Qubit 4. |
Researcher use of the Qubit using facility-provided consumables for RNA analysis |
$2/sample |
$2.25/sample |
Run on the ThermoFisher Qubit 4. |
Size selection on the BluePippin performed by facility technicians |
$25/sample |
$27.50/sample |
|
Nucleic Acid Extraction
Service |
ASU rate |
Nonprofit/other academic rate |
Notes |
DNA extraction |
$11/sample |
$12/sample |
DNA extraction using Qiagen/MoBio PowerSoil kit. |
RNA extraction |
$20/sample |
$22/sample |
RNA extraction using Qiagen RNeasy kit. |
RNA and DNA extraction |
$30/sample |
$35/sample |
Simultaneous DNA and RNA extraction from a single tissue sample. |
NGS Run Modules
Service |
ASU rate |
Nonprofit/other academic rate |
Notes |
MiSeq Nano |
Up to 1 million reads |
||
MiSeq Nano 2x150 |
$475/run |
$495/run |
Paired end run, each read 150bp |
MiSeq Nano 2x250 |
$525/run |
$550/run |
Paired end run, each read 250bp |
MiSeq v2
|
12-15 million reads |
||
MiSeq v2 2x150 |
$1400/run |
$1518/run |
Paired end run, each read 150bp |
MiSeq v2 2x250 |
$1580/run |
$1723/run |
Paired end run, each read 250bp |
MiSeq v3
|
20-22 million reads |
||
MiSeq v2 2x75 | $1225run | $1328/run | Paired end run, each read 75bp |
MiSeq v3 2x300 |
$2233/run |
$2233/run |
Paired end run, each read 300bp |
|
NGS Sequencing Reaads
Service |
ASU rate |
Nonprofit/other academic rate |
Notes |
Sequencing Lane
|
$6,515 | $6,515 | 2-3 Billion Reads |
Flow Cell
|
$25,260 | $25,260 | 8-10 Billion Reads |
Shipping Rate |
$50/project |
$50/project |
Priority Overnight shipment on dry ice |
NGS Bioinformatics Analyses
Service |
ASU rate |
Nonprofit/other academic rate |
Notes |
Microbiome Sequencing Demultiplexing |
$50/run |
$50/run |
|
Demultiplexing and Fastq File Generation |
$50/run |
$50/run |
Standard demultiplexing and fastq file generation, with QC analysis |
Please refer to the Bioinformatics Facility for analysis capabilities.