Rates

Service

Nonprofit/other academic rate

Notes

Clean and load

$3/sample

$3.10/sample

For samples already cycled with BigDye

PCR clean with sequencing

$5.35/sample

For samples needing PCR cleanup

PCR clean with sequencing, batch rate (49+)

$4.15/sample

$4.65/sample

Standard Sequencing

$4.25/sample

$4.75/sample

For clean samples

Standard Sequencing, batch rate (49+)

$3.50/sample

$4/sample

Service

Nonprofit/other academic rate

Notes

Single cell RNA-seq library preparation

$3,000/sample

$3,300/sample

On 10X Genomics platform

DNA library preparation

$165/sample
(1-12 samples)

$135/sample
(13-96 samples)

$105/sample
(97 or more samples)

$180/sample
(1-12 samples)

$150/sample
(13-96 samples)

$120/sample
(97 and more samples)

Synthesis of NGS libraries from DNA, 100-1000bp long

Microbiome 16S library preparation

$70/sample
(1-20 samples)

$65/sample
(21-49 samples)

$55/sample
(50-95 samples)

$35/sample
(96 or more samples)

$76.50/sample
(1-20 samples)

$71.50/sample
(21-49 samples)

$61.50/sample
(50-95 samples)

$41.50/sample
(96 or more samples)

Library preparation for microbiome sequencing, starting from genomic DNA

$85/sample
(1-20 samples)

$80/sample
(21-49 samples)

$70/sample
(50-95 samples)

$50/sample
(96 or more samples)

$91.50/sample
(1-20 samples)

$86.50/sample
(21-49 samples)

$76.50/sample
(50-95 samples)

$56.50/sample
(96 or more samples)

$85/sample
(1-20 samples)

$80/sample
(21-49 samples)

$70/sample
(50-95 samples)

$50/sample
(96 or more samples)

$91.50/sample
(1-20 samples)

$86.50/sample
(21-49 samples)

$76.50/sample
(50-95 samples)

$56.50/sample
(96 or more samples)

cDNA Synthesis only

$185/sample

$200/sample

cDNA synthesis from total RNA using SPIA random hexamers

RNA library preparation

$135/sample
(1-8 samples)

$105/sample
(9-24 samples)

$85/sample
(25 or more samples)

$147/sample
(1-8 samples)

$117/sample
(9-24 samples)

$97/sample
(25 or more samples)

NGS library construction from total RNA, rRNA depletion, or mRNA pulldown

mRNA pulldown

$55/sample

$60/sample

mRNA pull-down only

Bacterial rRNA depletion

$60/sample

$65/sample

removal of rRNA from bacterial samples

Service

Nonprofit/other academic rate

Notes

Covaris M220 shearing performed by facility technicians 

$16.50/sample

$16.50/sample

Researcher use of the Covaris M220 using consumables provided by the core

$13.20/sample

$13.20/sample

Researcher use of the Covaris M220 using researcher-provided consumables

$8/half hour

$8/half hour

Covaris M220 training

$90/training

$90/training

Hands on training – required before using Covaris independently. Includes consumables for 2 samples.

AgilentHaha TapeStation analysis performed by facility technicians

$15/sample

$17/sample

DNA and RNA fragment analysis.

Researcher use of the Agilent TapeStation using researcher-provided consumables

$9/half hour

$9/half hour

Agilent TapeStation training

$90/training

$90/training

Hands on training – required before using Agilent TapeStation independently. Includes consumables for two samples.

qPCR targeting Illumina adapter regions, in triplicate at three dilutions, with analysis run by facility staff

$25/sample

$27/sample

Run on the QuantStudio5.

Any researcher qPCR run with researcher-provided consumables

$15/plate

$16.50/plate

Run on the QuantStudio5.

QuantStudio5 training

$90/training

$90/training

Hands on training – required before using QuantStudio5 independently. Includes consumables for two samples.

Qubit using facility-provided consumables for DNA analysis

$2/sample

$2.25/sample

Run on the ThermoFisher Qubit 4.

Qubit using facility-provided consumables for RNA analysis

$2.50/sample

$3/sample

Run on the ThermoFisher Qubit 4.

Size selection on the BluePippin performed by facility technicians

$27.50/sample

$27.50/sample

Service

Nonprofit/other academic rate

Notes

DNA extraction

$15/sample

$17/sample

DNA extraction using Qiagen/MoBio PowerSoil kit.

RNA extraction

$25/sample

$27/sample

RNA extraction using Qiagen RNeasy kit.

Service

Nonprofit/other academic rate

Notes

MiSeq Nano

Up to 1 million reads

$575/run

$575/run

Paired end run, each read 150bp

MiSeq Nano 2x250

$650/run

Paired end run, each read 250bp

MiSeq v2

12-15 million reads

MiSeq v2 2x150

$1,525/run

$1,525/run

Paired end run, each read 150bp

MiSeq v2 2x250

$1,700/run

$1,700/run

Paired end run, each read 250bp

MiSeq v3

20-22 million reads

MiSeq v3 2x300

$2,375/run

$2,375/run

Paired end run, each read 300bp

Service

Nonprofit/other academic rate

Notes

S4 200 Sequencing Lane

S4 300 Sequencing Lane

SP 100 Sequencing Flow Cell

$3,399.80

SP 200 Sequencing Flow Cell

SP 300 Sequencing Flow Cell

SP 500 Sequencing Flow Cell

Shipping Rate

$25/project

$25/project

Priority Overnight shipment on dry ice

Service

Nonprofit/other academic rate

Notes

Microbiome Sequencing Demultiplexing

$65/run

$70/run

Demultiplexing and Fastq File Generation

$65/run

$70/run

Standard demultiplexing and fastq file generation, with QC analysis