Rates

Service

Academic/Non-Profit rate

Notes

Clean and load

$3.50/sample

$4.00/sample

For samples already cycled with BigDye.

PCR clean with sequencing

$6.50/sample

For samples needing PCR cleanup

PCR clean with sequencing, batch rate (49+)

$5.00/sample

$5.75/sample

Standard Sequencing

$5.00/sample

$5.75/sample

For clean samples

Standard Sequencing, batch rate (49+)

$4.25/sample

$5.00/sample

Service

Nonprofit/other academic rate

Notes

3' Single cell RNA-seq library preparation

$3500/sample

$3850/sample

On 10X Genomics platform

DNA library preparation

$192/sample
(1-12 samples)

$172/sample
(13-96 samples)

$152/sample
(97+ samples)

$208/sample
(1-12 samples)

$188/sample
(13-96 samples)

$168/sample
(97+ samples)

Synthesis of NGS libraries from DNA, 100-1000bp long

Microbiome 16S/18S/ITS library preparation

$90/sample
(1-20 samples)

$84/sample
(21-49 samples)

$73/sample
(50-95 samples)

$53/sample
(96 + samples)

$97/sample
(1-20 samples)

$91/sample
(21-49 samples)

$80/sample
(50-95 samples)

$60/sample
(96 + samples)

Library preparation for microbiome sequencing, starting from genomic DNA

cDNA Synthesis only

$215/sample

$231/sample

cDNA synthesis from total RNA using SPIA random hexamers

RNA library preparation

$160/sample
(1-8 samples)

$130/sample
(9-24 samples)

$105/sample
(25 or more samples)

$173/sample
(1-8 samples)

$143/sample
(9-24 samples)

$112/sample
(25 or more samples)

NGS library construction from total RNA, rRNA depletion, or mRNA pulldown

mRNA pulldown

$64/sample

$71.50/sample

mRNA pull-down only

Bacterial rRNA depletion

$70/sample

$75/sample

removal of rRNA from bacterial samples

Service

Nonprofit/other academic rate

Notes

Covaris M220 shearing performed by facility technicians 

$20/sample

$20/sample

Researcher use of the Covaris M220 using researcher-provided consumables

$19/hour

$19/hour

Covaris M220 training

$105/training

$105/training

Hands on training – required before using Covaris independently. Includes consumables for 2 samples.

Agilent TapeStation analysis performed by facility technicians

$18/sample

$20/sample

DNA and RNA fragment analysis.

Researcher use of the Agilent TapeStation using researcher-provided consumables

$25/hour

$25/hour

Agilent TapeStation training

$105/training

$105/training

Hands on training – required before using Agilent TapeStation independently. Includes consumables for two samples.

qPCR targeting Illumina adapter regions, in triplicate at three dilutions, with analysis run by facility staff

$30/sample

$32/sample

Run on the QuantStudio7.

Any researcher qPCR run with researcher-provided consumables

$18/plate

$20/plate

Run on the QuantStudio7.

QuantStudio5 training

$105/training

$105/training

Hands on training – required before using QuantStudio5 independently. Includes consumables for two samples.

Qubit using facility-provided consumables for DNA analysis

$3.00/sample

$3.50/sample

Run on the ThermoFisher Qubit 4.

Qubit using facility-provided consumables for RNA analysis

$4.00/sample

$4.50/sample

Run on the ThermoFisher Qubit 4.

Size selection on the BluePippin performed by facility technicians

$32/sample

$32/sample

Service

Nonprofit/other academic rate

Notes

DNA extraction

$18/sample

$20/sample

DNA extraction using Qiagen/MoBio PowerSoil kit.

RNA extraction

$30/sample

$32/sample

RNA extraction using Qiagen RNeasy kit.

Service

Nonprofit/other academic rate

Notes

MiSeq Nano

Up to 1 million reads

$572/run

$572/run

Paired end run, each read 150bp

MiSeq Nano 2x250

$750.75/run

Paired end run, each read 250bp

MiSeq v2

12-15 million reads

MiSeq v2 2x150

$1,765.50/run

$1,765.50/run

Paired end run, each read 150bp

MiSeq v2 2x250

$1,963.50/run

$1,963.50/run

Paired end run, each read 250bp

MiSeq v3

20-22 million reads

MiSeq v3 2x300

$2,744.50/run

$2,744.50/run

Paired end run, each read 300bp

Service

Nonprofit/other academic rate

Notes

NovaSeq X - 10B (300 cycle)

NovaSeq X - 25B (300 cycle)

$3250

Service

Nonprofit/other academic rate

Notes

Microbiome Sequencing Demultiplexing

$80/run

$85/run

Demultiplexing and Fastq File Generation

$80/run

$85/run

Standard demultiplexing and fastq file generation, with QC analysis