Rates

Service

Academic/Non-Profit rate

Notes

Clean and load

$3.25/sample

$3.50/sample

For samples already cycled with BigDye.

PCR clean with sequencing

$5.75/sample

For samples needing PCR cleanup

PCR clean with sequencing, batch rate (49+)

$4.50/sample

$4.75/sample

Standard Sequencing

$4.50/sample

$5.00/sample

For clean samples

Standard Sequencing, batch rate (49+)

$3.75/sample

$4.25/sample

Service

Nonprofit/other academic rate

Notes

Single cell RNA-seq library preparation

$3150/sample

$3465/sample

On 10X Genomics platform

DNA library preparation

$174/sample
(1-12 samples)

$142/sample
(13-96 samples)

$110/sample
(97+ samples)

$189/sample
(1-12 samples)

$157.5/sample
(13-96 samples)

$126/sample
(97+ samples)

Synthesis of NGS libraries from DNA, 100-1000bp long

Microbiome 16S library preparation

$73.5/sample
(1-20 samples)

$68.5/sample
(21-49 samples)

$58/sample
(50-95 samples)

$37/sample
(96 + samples)

$80.5/sample
(1-20 samples)

$75/sample
(21-49 samples)

$65/sample
(50-95 samples)

$44/sample
(96 + samples)

Library preparation for microbiome sequencing, starting from genomic DNA

$89.5/sample
(1-20 samples)

$84/sample
(21-49 samples)

$73.5/sample
(50-95 samples)

$52.5/sample
(96 + samples)

$96sample
(1-20 samples)

$91/sample
(21-49 samples)

$80.5/sample
(50-95 samples)

$60/sample
(96 + samples)

$90/sample
(1-20 samples)

$84/sample
(21-49 samples)

$73.5/sample
(50-95 samples)

$52.5/sample
(96 or more samples)

$96/sample
(1-20 samples)

$91/sample
(21-49 samples)

$80/sample
(50-95 samples)

$60/sample
(96 or more samples)

cDNA Synthesis only

$195/sample

$210/sample

cDNA synthesis from total RNA using SPIA random hexamers

RNA library preparation

$142/sample
(1-8 samples)

$112/sample
(9-24 samples)

$90/sample
(25 or more samples)

$155/sample
(1-8 samples)

$123/sample
(9-24 samples)

$102/sample
(25 or more samples)

NGS library construction from total RNA, rRNA depletion, or mRNA pulldown

mRNA pulldown

$58/sample

$65/sample

mRNA pull-down only

Bacterial rRNA depletion

$63/sample

$68/sample

removal of rRNA from bacterial samples

Service

Nonprofit/other academic rate

Notes

Covaris M220 shearing performed by facility technicians 

$18/sample

$18/sample

Researcher use of the Covaris M220 using consumables provided by the core

Researcher use of the Covaris M220 using researcher-provided consumables

Covaris M220 training

$95/training

$95/training

Hands on training – required before using Covaris independently. Includes consumables for 2 samples.

Agilent TapeStation analysis performed by facility technicians

$16/sample

$18/sample

DNA and RNA fragment analysis.

Researcher use of the Agilent TapeStation using researcher-provided consumables

Agilent TapeStation training

$95/training

$95/training

Hands on training – required before using Agilent TapeStation independently. Includes consumables for two samples.

qPCR targeting Illumina adapter regions, in triplicate at three dilutions, with analysis run by facility staff

$26.5/sample

$28.5/sample

Run on the QuantStudio5.

Any researcher qPCR run with researcher-provided consumables

$16/plate

$18/plate

Run on the QuantStudio5.

QuantStudio5 training

$90/training

$90/training

Hands on training – required before using QuantStudio5 independently. Includes consumables for two samples.

Qubit using facility-provided consumables for DNA analysis

$2.25/sample

$2.5/sample

Run on the ThermoFisher Qubit 4.

Qubit using facility-provided consumables for RNA analysis

$3/sample

$3.5/sample

Run on the ThermoFisher Qubit 4.

Size selection on the BluePippin performed by facility technicians

$29/sample

$29/sample

Service

Nonprofit/other academic rate

Notes

DNA extraction

$16/sample

$18/sample

DNA extraction using Qiagen/MoBio PowerSoil kit.

RNA extraction

$26.5/sample

$28.5/sample

RNA extraction using Qiagen RNeasy kit.

Service

Nonprofit/other academic rate

Notes

MiSeq Nano

Up to 1 million reads

$520/run

$520/run

Paired end run, each read 150bp

MiSeq Nano 2x250

$682.5/run

Paired end run, each read 250bp

MiSeq v2

12-15 million reads

MiSeq v2 2x150

$1605/run

$1605/run

Paired end run, each read 150bp

MiSeq v2 2x250

$1785/run

$1785/run

Paired end run, each read 250bp

MiSeq v3

20-22 million reads

MiSeq v3 2x300

$2495/run

$2495/run

Paired end run, each read 300bp

Service

Nonprofit/other academic rate

Notes

NovaSeq X - 1.5B (300 cycle)

NovaSeq X - 10B (300 cycle)

NovaSeq X - 25B (300 cycle)

$3110

Service

Nonprofit/other academic rate

Notes

Microbiome Sequencing Demultiplexing

$70/run

$75/run

Demultiplexing and Fastq File Generation

$70/run

$75/run

Standard demultiplexing and fastq file generation, with QC analysis