Rates

Service

Nonprofit/other academic rate

Notes

Clean and load

$3/sample

$3.10/sample

For samples already cycled with BigDye

PCR clean with sequencing

$5/sample

For samples needing PCR cleanup

PCR clean with sequencing, batch rate (49+)

$3.75/sample

$4.30/sample

Standard Sequencing

$3.70/sample

$4/sample

For clean samples

Standard Sequencing, batch rate (49+)

$3/sample

$3.30/sample

Service

Nonprofit/other academic rate

Notes

Single cell RNA-seq library preparation

$2,200/sample

$2,395/sample

On 10X Genomics platform

DNA library preparation

$150/sample
(1-12 samples)

$120/sample
(13-96 samples)

$95/sample
(97 or more samples)

$165/sample
(1-12 samples)

$135/sample
(13-96 samples)

$110/sample
(97 and more samples)

Synthesis of NGS libraries from DNA, 100-1000bp long

Microbiome library preparation

$65/sample
(1-20 samples)

$60/sample
(21-49 samples)

$50/sample
(50-95 samples)

$30/sample
(96 or more samples)

$71.50/sample
(1-20 samples)

$66.50/sample
(21-49 samples)

$56.50/sample
(50-95 samples)

$36.50/sample
(96 or more samples)

Library preparation for microbiome sequencing, starting from genomic DNA; can select either 16S (bacterial), 18S (eukaryotic), or ITS (fungal) regions

cDNA Synthesis only

$160/sample

$176/sample

cDNA synthesis from total RNA using SPIA random hexamers

RNA library preparation

$120/sample
(1-8 samples)

$90/sample
(9-24 samples)

$70/sample
(25 or more samples)

$132/sample
(1-8 samples)

$102/sample
(9-24 samples)

$82/sample
(25 or more samples)

NGS library construction from total RNA, rRNA depletion, or mRNA pulldown

mRNA pulldown

$40/sample

$44/sample

mRNA pull-down only

Bacterial rRNA depletion

$50/sample

$55/sample

removal of rRNA from bacterial samples

Service

Nonprofit/other academic rate

Notes

Covaris M220 shearing performed by facility technicians 

$15/sample

$16.50/sample

Researcher use of the Covaris M220 using consumables provided by the core

$12/sample

$13.20/sample

Researcher use of the Covaris M220 using researcher-provided consumables

$4/half hour

$6/half hour

Covaris M220 training

$75/training

$85/training

Hands on training – required before using Covaris independently. Includes consumables for 2 samples.

Agilent TapeStation analysis performed by facility technicians

$11/sample

$12/sample

Researcher use of the Agilent TapeStation using consumables provided by the core

$8/sample

$9/sample

Researcher use of the Agilent TapeStation using researcher-provided consumables

$4/half hour

$6/half hour

Agilent TapeStation training

$75/training

$85/training

Hands on training – required before using Agilent TapeStation independently. Includes consumables for two samples.

qPCR targeting Illumina adapter regions, in triplicate at three dilutions, with analysis run by facility staff

$20/sample

$22/sample

Run on the QuantStudio5.

Any researcher qPCR run with researcher-provided consumables

$15/plate

$16.50/plate

Run on the QuantStudio5.

QuantStudio5 training

$75/training

$85/training

Hands on training – required before using QuantStudio5 independently. Includes consumables for two samples.

Researcher use of the Qubit using facility-provided consumables for DNA analysis

$1.50/sample

$1.75/sample

Run on the ThermoFisher Qubit 4.

Researcher use of the Qubit using facility-provided consumables for RNA analysis

$2/sample

$2.25/sample

Run on the ThermoFisher Qubit 4.

Size selection on the BluePippin performed by facility technicians

$25/sample

$27.50/sample

Service

Nonprofit/other academic rate

Notes

DNA extraction

$11/sample

$12/sample

DNA extraction using Qiagen/MoBio PowerSoil kit.

RNA extraction

$20/sample

$22/sample

RNA extraction using Qiagen RNeasy kit.

RNA and DNA extraction

$30/sample

$35/sample

Simultaneous DNA and RNA extraction from a single tissue sample.

Service

Nonprofit/other academic rate

Notes

MiSeq Nano

Up to 1 million reads

$475/run

$495/run

Paired end run, each read 150bp

MiSeq Nano 2x250

$550/run

Paired end run, each read 250bp

MiSeq v2

12-15 million reads

MiSeq v2 2x150

$1400/run

$1518/run

Paired end run, each read 150bp

MiSeq v2 2x250

$1580/run

$1723/run

Paired end run, each read 250bp

MiSeq v3

20-22 million reads

MiSeq v3 2x300

$2233/run

$2233/run

Paired end run, each read 300bp

Service

Nonprofit/other academic rate

Notes

Sequencing Lane

Flow Cell

Shipping Rate

$50/project

$50/project

Priority Overnight shipment on dry ice

Service

Nonprofit/other academic rate

Notes

Microbiome Sequencing Demultiplexing

$50/run

$50/run

Demultiplexing and Fastq File Generation

$50/run

$50/run

Standard demultiplexing and fastq file generation, with QC analysis