Rates

Sanger Sequencing

Service

ASU rate

Nonprofit/other academic rate

Notes

Clean and load

$1/sample

$1.10/sample

For samples already cycled with BigDye

PCR clean with sequencing

$4.45/sample

$5/sample

For samples needing PCR cleanup

PCR clean with sequencing, batch rate (48+)

$3.75/sample

$4.30/sample

 

Standard Sequencing

$3.70/sample

$4/sample

For clean samples

Standard Sequencing, batch rate (48+)

$3/sample

$3.30/sample

 

 

NGS Sample Prep

Service

ASU rate

Nonprofit/other academic rate

Notes

Single cell RNA-seq library preparation

$1,950/sample

$2,145/sample

On 10X Genomics platform

DNA library preparation

$150/sample (1-12 samples)

$120/sample (13-96 samples)

$95/sample (97 or more samples)

$165/sample (1-12 samples)

$135/sample (13-96 samples)

$110/sample (97 and more samples)

Synthesis of NGS libraries from DNA, 100-1000bp long

Microbiome library preparation

$65/sample (1-20 samples)

$60/sample (21-49 samples)

$50/sample (50-95 samples)

$33/sample (96 or more samples)

$71.50/sample (1-20 samples)

$66.50/sample (21-49 samples)

$56.50/sample (50-95 samples)

$36.50/sample (96 or more samples)

Library preparation for microbiome sequencing, starting from genomic DNA; can select either 16S (bacterial), 18S (eukaryotic), or ITS (fungal) regions

ITS2 $10/sample $10/sample additional ITS (fungal) region option

cDNA Synthesis only

$160/sample

$176/sample

cDNA synthesis from total RNA using SPIA random hexamers

RNA library preparation

$120/sample (1-8 samples)

$90/sample (9-24 samples)

$70/sample (25 or more samples)

$132/sample (1-8 samples)

$102/sample (9-24 samples)

$82/sample (25 or more samples)

NGS library construction from total RNA, rRNA depletion, or mRNA pulldown

mRNA pulldown

$40/sample

$44/sample

mRNA pull-down only

Bacterial rRNA depletion

$50/sample

$55/sample

removal of rRNA from bacterial samples

Human/Mouse/Rate rRNA depletion $75/sample $82.50/sample removal of rRNA from human, mouse, or rat samples
Specialty rRNA depletion $120/sample $132/sample removal of rRNA from other species

High throughput small genome library preparation

$30/sample

$33/sample

NGS library construction for small genomes only, >100 samples

 

Instrument training and use

Service

ASU rate

Nonprofit/other academic rate

Notes

Covaris M220 shearing performed by facility technicians 

$15/sample

$16.50/sample

 

Researcher use of the Covaris M220 using consumables provided by the core

$12/sample

$13.20/sample

 

Researcher use of the Covaris M220 using researcher-provided consumables

$4/half hour

$6/half hour

 

Covaris M220 training

$75/training

$85/training

Hands on training – required before using Covaris independently. Includes consumables for 2 samples.

Agilent TapeStation analysis performed by facility technicians

$11/sample

$12/sample

 

Researcher use of the Agilent TapeStation using consumables provided by the core

$8/sample

$9/sample

 

Researcher use of the Agilent TapeStation using researcher-provided consumables

$4/half hour

$6/half hour

 

Agilent TapeStation training

$75/training

$85/training

Hands on training – required before using Agilent TapeStation independently. Includes consumables for two samples.

qPCR targeting Illumina adapter regions, in triplicate at three dilutions, with analysis run by facility staff

$20/sample

$22/sample

Run on the QuantStudio5.

Any researcher qPCR run with researcher-provided consumables

$15/plate

$16.50/plate

Run on the QuantStudio5.

QuantStudio5 training

$75/training

$85/training

Hands on training – required before using QuantStudio5 independently. Includes consumables for two samples.

Researcher use of the Qubit using facility-provided consumables for DNA analysis

$1.50/sample

$1.75/sample

Run on the ThermoFisher Qubit 4.

Researcher use of the Qubit using facility-provided consumables for RNA analysis

$2/sample

$2.25/sample

Run on the ThermoFisher Qubit 4.

Size selection on the BluePippin performed by facility technicians

$25/sample

$27.50/sample

 

Nucleic Acid Extraction

Service

ASU rate

Nonprofit/other academic rate

Notes

DNA extraction

$11/sample

$12/sample

DNA extraction using Qiagen/MoBio PowerSoil kit.

RNA extraction

$20/sample

$22/sample

RNA extraction using Qiagen RNeasy kit.

RNA and DNA extraction

$30/sample

$35/sample

Simultaneous DNA and RNA extraction from a single tissue sample.

 

NGS Run Modules

Service

ASU rate

Nonprofit/other academic rate

Notes

MiSeq Nano

Up to 1 million reads

MiSeq Nano 2x150

$450/run

$495/run

Paired end run, each read 150bp

MiSeq Nano 2x250

$500/run

$550/run

Paired end run, each read 250bp

 

MiSeq v2

 

12-15 million reads

MiSeq v2 2x150

$1380/run

$1518/run

Paired end run, each read 150bp

MiSeq v2 2x250

$1566/run

$1723/run

Paired end run, each read 250bp

 

MiSeq v3

 

20-22 million reads

MiSeq v2 2x75 $1207.50/run $1328/run Paired end run, each read 75bp

MiSeq v3 2x300

$2030/run

$2233/run

Paired end run, each read 300bp

 

NextSeq Mid Output

 

130 million reads

NextSeq 2x75 Mid

$1450/run

$1595/run

Paired end run, each read 75bp

NextSeq 2x150 Mid

$2310/run

$2541/run

Paired end run, each read 150bp

 

NextSeq High Output

 

400 million reads

NextSeq 1x75 High

$1976/run

$2174/run

Single end run, 75bp

NextSeq 2x75 High

$3762/run

$4138/run

Paired end run, each read 75bp

NextSeq 2x150 High

$6032/run

$6636/run

Paired end run, each read 150bp

NGS Sequencing Reaads

Service

ASU rate

Nonprofit/other academic rate

Notes

Sequencing Reads

$53/10 Million Reads 

$53/10 Million Reads

Reads can be requested in increments of 10 Million

Shipping Rate

$50/project

$50/project

Priority Overnight shipment on dry ice

NGS Bioinformatics Analyses

Service

ASU rate

Nonprofit/other academic rate

Notes

Microbiome Sequencing Demultiplexing

$50/run

$50/run

 

Demultiplexing and Fastq File Generation

$50/run

$50/run

Standard demultiplexing and fastq file generation, with QC analysis

 

Please refer to the Bioinformatics Facility for analysis capabilities.